Application of L-amino acid in determination of Huperzine A by high performance liquid chromatography

作者：Pan J; Jin RS^*; Hu XQ

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2006, 836(1-2): 108-110.

DOI：10.1016/j.jchromb.2006.03.018

• Huperzine A
• HPLC

A selective HPLC method for determination of Huperzine A in Huperzia serrata Extract has been developed and validated. Huperzine A was dissolved in 0.01 mol/L HC1, chromatographed on an AgilentZorbax SB-C18 (150 mm x 4.6 mm i.d., mu m) column, with a mobile phaseconsisting of methanol-1 mM (L)-Lysine water solution (50:50, v/v), and detected at 310 nm. The UV peak areas were used for quantitation of Huperzine A content. The correlation coefficient (R-2) of the calibration was 0.9999 over the range of 1-25 mu g/ml and intra- and interday precision over this range were not more than 2%. The method was successfully applied to characterize and determine the Huperzine A in Huperzia serrata Extract.

• 出版日期2006-5-19
• 单位合肥工业大学