Assessing LINE-1 retrotransposition activity in neuroblastoma cells exposed to extremely low-frequency pulsed magnetic fields

作者：Del Re Brunella^*; Marcantonio Pamela; Gavoci Entele; Bersani Ferdinando; Giorgi Gianfranco

来源：Mutation Research: Genetic Toxicology and Environmental Mutagenesis , 2012, 749(1-2): 76-81.

DOI：10.1016/j.mrgentox.2012.07.004

• Pulsed magnetic field
• Extremely low-frequency magnetic field
• Retrotransposition
• LINE-1
• Neuroblastoma
• Genotoxicity

Mobile genetic elements represent an important source of mutation and genomic instability, and their activity can be influenced by several chemical and physical agents. %26lt;br%26gt;In this research we address the question whether exposure to extremely low-frequency pulsed magnetic fields (EMF-PMF) could affect the mobility of the human LINE-1(RP) retrotransposon. To this purpose, an in vitro retrotransposition assay was used on human neuroblastoma BE(2) cells exposed for 48 h to 1 mT, 50 Hz PMF, or sham-exposed. Moreover, since it is well known that retrotransposition causes DNA double-strand breaks (DSB), an estimation of gamma-H2AX foci, which is a marker of DNA DSB, was carried out on PMF- and sham-exposed samples. The results show that PMF-exposed cells had a lower number of both retrotransposition events and DNA DSB compared with sham-exposed samples. These results suggest that exposure to PMF can interfere with retrotransposition activity by inducing a decrease of retrotransposition events.

• 出版日期2012-12-12