Muscone Protects Vertebral End-plate Degeneration by Antiinflammatory Property

作者：Liang, Qian-Qian; Zhang, Min; Zhou, Quan; Shi, Qi; Wang, Yong-Jun^*

来源：Clinical Orthopaedics and Related Research, 2010, 468(6): 1600-1610.

DOI：10.1007/s11999-009-1079-0

• INTERVERTEBRAL DISC DEGENERATION
• NF-KAPPA-B
• ARTICULAR CHONDROCYTES
• NITRIC-OXIDE
• INFLAMMATORY MEDIATORS
• DOWN-REGULATION
• IN-VITRO
• EXPRESSION
• CARTILAGE
• INTERLEUKIN-1

Most chronic neck pain is the result of degeneration of the cervical spine. IL-1 beta may play an important role in intervertebral disc degeneration. This being the case, inhibiting IL-1 beta could provide a therapeutic approach for reducing or preventing disc degeneration. Muscone reportedly relieves pain and suppresses inflammation. Therefore, we asked whether muscone, a potent antiinflammatory agent, could reduce proinflammatory cytokines in vitro (end-plate cartilage cultures) and end-plate degeneration in vivo (a rat model that induces intervertebral disc degeneration). In vitro, muscone reversed IL-1 beta-induced upregulation of IL-1 beta, tumor necrosis factor alpha, cyclooxygenase 2, inducible nitric oxide synthase, matrix metalloproteinase 13, aggrecanase 2, and nitric oxide and downregulation of Col2 alpha 1 and aggrecan. Pretreatment with muscone (6.25, 12.5, 25 mu mol/L) inhibited the IL-1 beta-induced phosphorylation of extracellular signal-regulated kinases 1/2 and c-Jun N-terminal kinase in a dose-dependent manner. In vivo, muscone inhibited the expression of prostaglandin E2, 6-keto-prostaglandin F1 alpha, IL-1 beta, and tumor necrosis factor alpha and recovered the structural distortion of the degenerative disc. Our findings suggest muscone is a promising agent for treating intervertebral disc degeneration through its antiinflammatory effects.

• 出版日期2010-6
• 单位上海中医药大学