An Organic Sol-Gel Film as Modifier to Construct Biosensor

作者：Wu Jian Feng; Xu Miao Qing; Zhao Guang Chao^*

来源：Electroanalysis, 2009, 21(2): 196-200.

DOI：10.1002/elan.200804460

• Organic sol-gel
• Hemoglobin
• Biosensor
• Hydrogen peroxide

A new amperometric biosensor for hydrogen pet-oxide (H(2)O(2)) was developed by adsorbing hemoglobin (Hb) on an organic sol-gel film. The organic sol-gel was prepared using resorcinol and formaldehyde as monomers. This sol-gel film shows a biocompatible microenvironment for retaining the native activity of the adsorbed Hb. The direct electron transfer between Hb and electrode is achieved. Hb adsorbed oil the film shows an enzyme-like catalytic activity to the reduction of H(2)O(2). The reduction peak currents are proportional linearly to the concentration of hydrogen peroxide in the range of 6 x 10(-8) to 3.6 x 10(-6) M, with a detection limit of 2.4 x 10(-8) M (S/N = 3). This research enlarges the applications of organic sol-gel materials in biosensor field.

• 出版日期2009-1
• 单位安徽师范大学