Analysis of 3-O-sulfo group-containing heparin tetrasaccharides in heparin by liquid chromatography-mass spectrometry

作者：Li Guoyun; Yang Bo; Li Lingyun; Zhang Fuming; Xue Changhu; Linhardt Robert J^*

来源：Analytical Biochemistry, 2014, 455: 3-9.

DOI：10.1016/j.ab.2014.02.033

• 3-O-SuIfo group
• Tetrasaccharides
• Porcine intestinal heparin
• Anticoagulant activity
• RPIP-LC-MS
• Heparin lyase 2

Complete heparin digestion with heparin lyase 2 affords a mixture of disaccharides and resistant tetrasaccharides with 3-O-sulfo group-containing glucosamine residues at their reducing ends. Quantitative online liquid chromatography-mass spectrometric analysis of these resistant tetrasaccharides is described in this article. The disaccharide and tetrasaccharide compositions of seven porcine intestinal heparins and five low-molecular-weight heparins were analyzed by this method. These resistant tetrasaccharides account for from 5.3 to 7.3 wt% of heparin and from 6.2 to 8.3 wt% of low-molecular-weight heparin. Because these tetrasaccharides are derived from heparin%26apos;s antithrombin III-binding sites, we examined whether this method could be applied to estimate the anticoagulant activity of heparin. The content of 3-O-sulfo group-containing tetrasaccharides in a heparin correlated positively (r = 0.8294) to heparin%26apos;s anticoagulant activity.

• 出版日期2014-6-15
• 单位中国海洋大学