A new rhodamine B-based lysosomal pH fluorescent indicator

作者：Lv Hong Shui; Huang Shu Ya; Zhao Bao Xiang^*; Miao Jun Ying

来源：Analytica Chimica Acta, 2013, 788: 177-182.

DOI：10.1016/j.aca.2013.06.038

• Rhodamine B
• Fluorescent probe
• Intracellular pH
• Living cells
• Lysosomes

We designed and synthesized a new pH fluorescent probe, RCE, based on structural changes of rhodamine dye at different pH values. The probe exhibits high selectivity, high sensitivity and quick response to acidic pH, as well as low cytotoxicity, excellent photostability, reversibility and cell membrane permeability. Fluorescence intensity at 584 nm was increased more than 150-fold within pH range 7.51-3.53. This probe has pK(a) value 4.71, which is valuable for studying acidic organelles. Because of its long absorption and emission wavelengths, RCE can avoid associated cell damage. The probe can selectively stain lysosomes and monitor lysosomal pH changes in living cells.

• 出版日期2013-7-25
• 单位山东大学