A F-19 NMR probe for the detection of beta-galactosidase: simple structure with low molecular weight of 274.2, "turn-on'' signal without the background, and good performance applicable in cancer cell line

作者：Hu, Jie; Wu, Qiong; Cheng, Kai; Xie, Yujun; Li, Conggang^*; Li, Zhen^*

来源：Journal of Materials Chemistry B, 2017, 5(24): 4673-4678.

DOI：10.1039/c7tb00616k

• GENE REPORTER MOLECULES
• MAGNETIC-RESONANCE
• OVARIAN-CANCER
• VISUALIZATION
• GROWTH
• MRI

Based on the efficient cleavage reaction of the C-O ether bond triggered by beta-gal selectively, FB-beta Gal, with good water-solubility, low toxicity, high specificity, excellent water-solubility and high biocompatibility, was prepared, which could report the presence of trace beta-gal quickly and conveniently by a significant change in the F-19 NMR spectra without any background noise. The successful application of FB-beta Gal for the detection of beta-gal in living Escherichia coli, HeLa cells and OVCAR-3 cells quantitatively makes it a promising candidate for practical application in related fields.

• 出版日期2017-6-28
• 单位武汉大学; 中国科学院武汉物理与数学研究所