Activation of matrix metalloproteinase-2 in human breast cancer cells overexpressing cyclooxygenase-1 or-2

作者：Takahashi Y^*; Kawahara F; Noguchi M; Miwa K; Sato H; Seiki M; Inoue H; Tanabe T; Yoshimoto T

来源：FEBS Letters, 1999, 460(1): 145-148.

DOI：10.1016/S0014-5793(99)01328-9

• cyclooxygenase-1
• cyclooxygenase-2
• breast cancer cell
• cancer metastasis
• matrix metalloproteinase

Human breast cancer cell line Hs578T was stably transfected with cDNA for cyclooxygenase-1 or -2, When the cells overexpressing cyclooxygenase-1 or -2 mere stimulated with concanavalin A, the processing of matrix metalloproteinase-2 was observed with the aid of gelatin zymography, This processing was not seen in mock-transfected and original cells which did not express detectable cyclooxygenase activity. Furthermore, Northern blotting shelved 8-13 fold induction of membrane-type 1 matrix metalloproteinase,which processed matrix metalloproteinase-2 in the cells expressing cyclooxygenases. These findings suggest that both isoforms of cyclooxygenase mediate the processing of matrix metalloproteinase-2 through induction of membrane-type 1 metalloproteinase in breast cancer cells.

• 出版日期1999-10-22