科研之友
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摘要
Pituitary tumors are the most common type of cancer within the central nervous system. In the present study, the expression levels of mucin 1 (Muc1) were examined in invasive and non-invasive pituitary tumor samples, and the results of immunohistochemical staining and Western blot analysis demonstrated marked positive expression of Muc1. In addition, Muc1 + polyinosinic: polycytidylic acid (poly I:C) was found to stimulate the expression levels of the surface molecules cluster of differentiation (CD) 40, CD83 and CD80, and HLA-DRm and decreased the expression of CD14 in the dendritic cells, determined using fluorescence-activated cell sorting. The secretions of interleukin (IL)-6, tumor necrosis factor-a and IL-1 beta cytokines were also significantly induced, in a dose-dependent manner. In in vivo experiments, a higher percentage of CD3(+)CD4(+) T lymphocytes was detected, and the levels of interferon-gamma and IL-2 in the splenocytes were also upregulated. Furthermore, the combination treatment of Muc1 with poly I: C increased anti-Muc1 IgM and anti-Muc1 IgG titers, and altered the balance of IgG2a and IgG1, all of which increased the T helper (Th)1 polarized immune response. Thus, the tumor antigen, Muc1, with poly I: C may produce potent protective effects, which polarize immune responses towards Th1, and elicit antitumor immunity to inhibit the progression of pituitary tumors.
- 出版日期2016-4
- 单位滨州医学院
