Association of insulin sensitivity and glucose tolerance with the c.825C > T variant of the G protein beta-3 subunit gene

作者：Kopf Daniel^*; Cheng Li S C; Blandau Petra; Hsueh Willa; Raffel Leslie J; Buchanan Thomas A; Xiang Anny H; Davis Richard C; Rotter Jerome I; Lehnerta Hendrik

来源：Journal of Diabetes and Its Complications, 2008, 22(3): 205-209.

DOI：10.1016/j.jdiacomp.2006.12.005

• insulin resistance
• GNB3
• euglycemic clamp
• blood pressure
• metabolic syndrome

The risk of macrovascular complications of diabetes mellitus is greatly enhanced by the presence of high blood pressure. In addition, hypertension and diabetes share insulin resistance as a common pathophysiological mechanism. Despite evidence for a common molecular genetic background of insulin resistance, glucose intolerance, and hypertension, few candidate genes have been shown to influence all of these features simultaneously. We examined the association of insulin sensitivity with,the c.825C>T variant of the g-protein beta-3 subunit (GNB3), a candidate gene of hypertension, in families of Mexican-American hypertensive patients. Methods: One hundred eighty subjects enrolled in a family study of Mexican-American hypertensive patients were recruited from hypertension clinics in Los Angeles. Subjects underwent pretreatment blood pressure recording, an oral glucose tolerance test, euglycemic hyperinsulinemic clamp, and anthropometric measurements. DNA from peripheral blood leukocytes was genotyped by polymerase chain reaction and restriction enzyme digest with BseD1 (GNB). Statistical analysis was performed by transmission disequilibrium testing. Results: In carriers of the T-allele, blood glucose was significantly lower [(mean+S.D.) fasting: 96.7+22.9 vs. 106.7+51.7 mg/dl, P=.009; oral glucose tolerance test (oGTT) 120 min: 131.7+48.7 vs. 137.8+64.9 mg/dl, P=.036], and insulin sensitivity was significantly higher (229.0+108.7 vs. 188.5+94.2 mg/kg per minute, P=.037) than in homozygous carriers of the C-allele. Blood pressure did not differ significantly between the phenotypes. Conclusion: In a Mexican-American hypertensive population, we found evidence for higher insulin sensitivity in carriers of the T allele of the c.825C>T variant of GNB3.

• 出版日期2008-6
• 单位上海市精神卫生中心