Adenovirus type 3 induces platelet activation in vitro

作者：Jin, Ying-Yu; Yu, Xiu-Nan; Qu, Zhang-Yi^*; Zhang, Ai-Ai; Xing, Yu-Ling; Jiang, Li-Xin; Shang, Lei; Wang, Ying-Chen

来源：Molecular Medicine Reports, 2014, 9(1): 370-374.

DOI：10.3892/mmr.2013.1805

• adenovirus
• platelet
• CD62P
• CD41a
• fibrinogen
• adenovirus type 3

In the present study, we aimed to investigate platelet activation induced by adenovirus type 3 (HAdV3) in vitro. Platelet-rich plasma (PRP) or whole blood was incubated with or without HAdV at various concentrations. Platelet aggregation, platelet counting, fibrinogen and expression of platelet membrane antigens (CD41a and CD62P) were determined following incubation with HAdV for different periods of time. The results demonstrated that HAdV at the concentrations of 10(9)-10(11) vp/ml enhanced adenosine diphosphate (ADP) or ristocetin-induced platelet aggregation, however did not alter the platelet count. Infection with HAdVs also reduced fibrinogen level. P-selectin and CD41a appeared rapidly on the surface after platelets were incubated with HAdVs in vitro for 30 min. In conclusion, HAdVs may induce activation of platelets and lead to a pre-thrombotic state of peripheral blood. This finding may aid in the development of measures to prevent severe HAdV infection.

• 出版日期2014-1
• 单位哈尔滨医科大学