Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

作者：Mendes Natalia J; Cruz Vanessa P; Ashikaga Fernando Y; Camargo Samia M; Oliveira Claudio; Piercy Andrew N; Burgess George H; Coelho Rui; Santos Miguel N; Mendonca Fernando F^*; Foresti Fausto

来源：PeerJ, 2016, 4: e2205.

DOI：10.7717/peerj.2205

• High-throughput sequencing
• Galeocerdo cuvier
• Shark
• Microsatellites
• Population structure

The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and it is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study, we identified nine microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci were assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was successful at most loci for other species such as Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.

• 出版日期2016-8-30
• 单位Univ Estadual Paulista