Association of detoxification enzymes with butene-fipronil in larvae and adults of Drosophila melanogaster

作者：Arain, Muhammad Shahid^*; Shakeel, Muhammad; Elzaki, Mohammed Esmail Abdalla; Farooq, Muhammad; Hafeez, Muhammad; Shahid, Muhammad Rafiq; Shah, Syed Ali Haider; Khan, Fawad Zafar Ahmad; Shakeel, Qaiser; Salim, Abdalla Markaz Abdalla; Li, Guo-Qing^*

来源：Environmental Science and Pollution Research, 2018, 25(10): 10006-10013.

DOI：10.1007/s11356-018-1202-4

• Butene-fipronil
• Detoxification enzymes
• Drosophila melanogaster
• Synergists

Insecticide resistance is a major challenge in successful insect pest control as the insects have the ability to develop resistance to various widely used insecticides. Butene-fipronil is a novel compound with high toxicity to insects and less toxicity to the non-target organisms. In the present study, the effect of butene-fipronil alone and in combination with three enzyme inhibitors, piperonyl butoxide (PBO), diethyl maleate (DEM), and triphenyl phosphate (TPP), was carried out on larvae and adults of Drosophilia melanogaster. Our results indicated that the co-toxicity indices of butene-fipronil + PBO, butene-fipronil + TPP, and butene-fipronil + DEM mixtures were 437.3, 335.0, and 210.3, respectively, in the second-instar larvae, while 186.6, 256.2, and 238.5, respectively, in the adults, indicating synergistic effects. Interestingly, butene-fipronil increased the expression of CYP28A5 in the larvae; CYP9F2, CYP304A1, CYP28A5, and CYP318A1 in the female adults; and CYP303A1 andCYP28A5 in the male adults. Furthermore, high-level expression of Est-7 was observed in the female adults compared to larvae and male adults. Our results suggest that there is no difference in butene-fipronil metabolism in larvae and male and female adults of D. melanogaster.

• 出版日期2018-4
• 单位华南农业大学; 南京农业大学; 华中农业大学