Activation of macrophages by the ophiopogon polysaccharide liposome from the root tuber of Ophiopogon japonicus

作者：Sun, Wenjing; Hu, Wenjie; Meng, Kai; Yang, Lumiao; Zhang, Weimin; Song, Xiaoping^*; Qu, Xiaohao; Zhang, Yueyang; Ma, Lin; Fan, Yunpeng^*

来源：International Journal of Biological Macromolecules, 2016, 91: 918-925.

DOI：10.1016/j.ijbiomac.2016.06.037

• Ophiopogon polysaccharide liposome
• Peritoneal macrophages
• Phagocytosis

This study evaluated the immunomodulatory effects of ophiopogon polysaccharide liposome (OPL) on macrophages in vitro. The phagocytic activity, the secretion of nitric oxide (NO) and inducible nitric oxide synthase (iNOS), the level of cytokines, chemokines and the expression of CD14 and MHC-II costimulatory molecules were measured. Results showed that OPL could significantly improve the phagocytic activity and the level of IL-1 beta, TNF-alpha, MCP-1 and MIP-1 beta, promote the secretion of NO and iNOS, and enhance the expression of CD14 and MHC-II costimulatory molecules in the peritoneal macrophages of mice compared with ophiopogon polysaccharide (OP). Altogether, these results suggested that OPL could activate macrophages, and the efficacy was significantly superior to OP. Therefore, OPL would be exploited in a potent immunomodulators. Moreover, it also provided the theoretical basis for further studying the mechanism of OPL on improving the immune response.

• 出版日期2016-10
• 单位西北农林科技大学; 江苏省农业科学院