Novel calcineurin interacting protein-2: the functional characterization of CNP-2 in Caenorhabditis elegans

作者：Cai Xianglan; Ko Kyung Min; Singaravelu Gunasekaran; Ann Joohong^*

来源：BMB Reports, 2008, 41(6): 455-460.

• calcineurin (Cn)
• calcineurin binding protein
• C. elegans
• CNP-2
• TAX-6

Calcineurin (Cn) is a serine/threonine phosphatase implicated in a wide variety of biological responses. To identify proteins that mediate Cn signaling pathway effects, we used yeast two-hybrid assays to screen for Cn interacting proteins, discovering a protein encoded by the gene, cnp-2 (Y46G5A.10). Utilizing serially deleted forms of Cn as baits, we demonstrated that the catalytic domain of Cn (TAX-6) binds with CNP-2, and this physical interaction was able to be reconstituted in vitro, supporting our yeast two-hybrid results. cnp-2 is a nematode-specific novel gene found in C elegans as well as its closest relative, C. briggsae. CNP-2 was strongly expressed in the intestine of C elegans. To study the function of cnp-2, we performed cnp-2 RNAi knock-down and characterized phenotypes associated with Cn mutants. However, no gross defects were revealed in these RNAi experiments. CNP-2 was proven to be a Cn binding protein; however, its role remains to be elucidated.

• 出版日期2008-6-30