A specific cell-penetrating peptide induces apoptosis in SKOV3 cells by down-regulation of Bcl-2

作者：Ma Chuying; Yin Guangfu^*; You Fei; Wei Yan; Huang Zhongbing; Chen Xianchun; Yan Danhong

来源：Biotechnology Letters, 2013, 35(11): 1791-1797.

DOI：10.1007/s10529-013-1263-x

• Cell-penetrating
• Down-regulation of Bcl-2
• Early-stage apoptosis
• Peptide TLS
• SKOV3 cells

Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptake process is energy-dependent. It inhibited the proliferation of SKOV3 cells and induced early-stage apoptosis by down-regulation of Bcl-2 expression mediated through a caspase-dependent pathway. The synergistic anti-proliferative effects of the peptide TLS and doxorubicin on SKOV3 cells were further investigated. Taken together, TLS, acting as a combination of a targeted ligand and a therapeutic agent, was a promising candidate for the development of peptide-based therapies in ovarian cancer.

• 出版日期2013-11
• 单位四川大学