A single 60-min bout of peristaltic pulse external pneumatic compression transiently upregulates phosphorylated ribosomal protein s6

作者：Martin J S; Kephart W C; Mobley C B; Wilson T J; Goodlett M D; Roberts M D

来源：Clinical Physiology and Functional Imaging, 2017, 37(6): 602-609.

DOI：10.1111/cpf.12343

• Akt-mTOR signalling
• external pneumatic compression
• phosphorylation
• rps6
• skeletal muscle

We investigated whether a single 60-min bout of whole leg, peristaltic pulse external pneumatic compression (EPC) altered select growth factor-related mRNAs and/or various phospho(p)-proteins related to cell growth, proliferation, inflammation and apoptosis signalling (e.g. Akt-mTOR, Jak-Stat). Ten participants (8 males, 2 females; aged 22.2 +/- 0.4years) reported to the laboratory 4h post-prandial, and vastus lateralis muscle biopsies were obtained prior to (PRE), 1h and 4h post-EPC treatment. mRNA expression was analysed using real-time RT-PCR and phosphophorylated and cleaved proteins were analysed using an antibody array. No changes in selected growth factor-related mRNAs were observed following EPC. All p-proteins significantly altered by EPC decreased, except for p-rps6 (Ser235/236) which increased 31% 1h post-EPC compared to PRE levels (P=0.016). Notable decreases also included p-BAD (Ser112; -28%, P=0.004) at 4h post-EPC compared to PRE levels. In summary, an acute bout of EPC transiently upregulates p-rps6 as well as affecting other markers in the Akt-mTOR signalling cascade. Future research should characterize whether chronic EPC application promotes alterations in lower-limb musculature and/or enhances exercise-induced training adaptations.

• 出版日期2017-11