科研之友
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摘要
Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. Here we show that colonic epithelial model HCT116 cells respond to Trypanosoma cruzi infection by secreting defensin alpha-1, which reduces infection. We also report the early effects of defensin alpha-1 on invasive trypomastigotes that involve damage of the flagellar structure to inhibit parasite motility and reduce cellular infection. Short exposure of defensin alpha-1 to trypomastigotes shows that defensin alpha-1 binds to the flagellum, resulting in flagellar membrane and axoneme alterations, followed by breaking of the flagellar membrane connected to the trypanosome body, leading to detachment and release of the parasite flagellum. In addition, defensin alpha-1 induces a significant reduction in parasite motility in a peptide concentration-dependent manner, which is abrogated by anti-defensin alpha-1 IgG. Preincubation of trypomastigotes with a concentration of defensin alpha-1 that inhibits 50% trypanosome motility significantly reduced cellular infection by 80%. Thus, human defensin alpha-1 is an innate immune molecule that is secreted by HCT116 cells in response to T. cruzi infection, inhibits T. cruzi motility, and plays an important role in reducing cellular infection. This is the first report showing a novel cellular innate immune response to a human parasite by secretion of defensin alpha-1, which neutralizes the motility of a human parasite to reduce cellular infection. The mode of activity of human defensin alpha-1 against T. cruzi and its function may provide insights for the development of new antiparasitic strategies.
- 出版日期2013-11
