Neurotoxic activity of Gln49 phospholipase A(2) from Gloydius ussuriensis snake venom

作者：Chang Yan; Li Yingxue; Bao Yongming^*; An Lijia

来源：Journal of Applied Toxicology, 2007, 27(5): 447-452.

DOI：10.1002/jat.1222

• snake venom
• phospholipase A(2)
• Gloydius ussuriensis
• neurotoxin
• hot-plate test
• analgesic activity

A novel neurotoxic protein phospholipase A(2) (PLA(2)), molecular weight 13 881.83 Da, has been isolated from snake venom of Gloydius ussuriensis, named as Gln49-PLA(2), which shows weak lethal toxic, myotoxic and apparent anticoagulant activity, but lacks phospholipase activity. The Gln49-PLA(2) obviously induced an increase of the pain threshold in intoxicated 615 mice compared with the control group, suggesting it is a neurotoxin. Hot-plate tests also showed that its analgesic activity was dose-dependent, and naloxone antagonized the analgesic effect, implying the mechanism of action of Gln49-sPLA(2) is correlated with opioid receptors. Electrophysiology studies revealed decreases in the action potential and the nerve conduction velocity in isolated hoptoad (Bufo bufo gargarizans Cantor) sciatic nerve, indicating Gln49-PLA(2) most probably had effects on ion channels.

• 出版日期2007-10
• 单位大连理工大学