Urinary Concentrations of Steroids in Bulls under Anabolic Treatment by Revalor-XS (R) Implant

作者：Biancotto Giancarlo; Stella Roberto; Barrucci Federica; Lega Francesca; Angeletti Roberto

来源：Journal of Analytical Methods in Chemistry, 2016, 2016: 8013175.

DOI：10.1155/2016/8013175

<jats:p>Despite the European ban of using anabolics in food-producing animals, growth promoters might still be illegally used in the European Union. To control the food chain and guarantee consumers’ health, there is a need of highly sensitive analytical methods for the identification of marker residues of such treatments. In the present study, a group of bulls (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">16</mml:mn></mml:math>) received trenbolone acetate (200 mg) and estradiol (40 mg) by a commercial ear implant during a time range of 71 days, and a second group (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">16</mml:mn></mml:math>) was kept for control. The aim of the research was to measure the residual urinary concentrations of the administered drugs (<jats:italic>β</jats:italic>-trenbolone and<jats:italic>β</jats:italic>-estradiol), their main metabolites (<jats:italic>α</jats:italic>-trenbolone and<jats:italic>α</jats:italic>-estradiol), and possible alterations of the urinary profile of other endogenous hormones metabolically related. The analytical method was based on liquid chromatography-tandem mass spectrometry. Results showed average urinary concentrations of<jats:italic>α</jats:italic>-trenbolone and<jats:italic>α</jats:italic>-estradiol during treatment in the range of (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mn fontstyle="italic">0.81</mml:mn><mml:mo>÷</mml:mo><mml:mn fontstyle="italic">2.1</mml:mn></mml:math>) ng mL<jats:sup>−1</jats:sup>and (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mn fontstyle="italic">0.96</mml:mn><mml:mo>÷</mml:mo><mml:mn fontstyle="italic">4.4</mml:mn></mml:math>) ng mL<jats:sup>−1</jats:sup>, respectively, whereas<jats:italic>β</jats:italic>-trenbolone and<jats:italic>β</jats:italic>-estradiol exhibit urinary concentrations lower than 0.22 ng mL<jats:sup>−1</jats:sup>in both cases. Data obtained from the urinary profiles of endogenous steroids indicate that they could be useful to indirectly detect the ongoing treatment.</jats:p>

• 出版日期2016