A Simple Method to Measure Renal Function in Swine by the Plasma Clearance of Iohexol

作者：Luis Lima Sergio; Garcia Contreras Consolacion; Vazquez Gomez Marta; Astiz Susana; Carrara Fabiola; Gaspari Flavio; Negrin Mena Natalia; Jimenez Sosa Alejandro; Jimenez Hernandez Hugo; Gonzalez Bulnes Antonio; Porrini Esteban^*

来源：International Journal of Molecular Sciences, 2018, 19(1): 232.

DOI：10.3390/ijms19010232

• renal function
• iohexol plasma clearance
• swine model

There is no simple method to measure glomerular filtration rate (GFR) in swine, an established model for studying renal disease. We developed a protocol to measure GFR in conscious swine by using the plasma clearance of iohexol. We used two groups, test and validation, with eight animals each. Ten milliliters of iohexol (6.47 g) was injected into the marginal auricular vein and blood samples (3 mL) were collected from the orbital sinus at different points after injection. GFR was determined using two models: two-compartment (CL2: all samples) and one-compartment (CL1: the last six samples). In the test group, CL1 overestimated CL2 by similar to 30%: CL2 = 245 +/- 93 and CL1 = 308 +/- 123 mL/min. This error was corrected by a first-order polynomial quadratic equation to CL1, which was considered the simplified method: SM = -47.909 + (1.176xCL1) - (0.00063968xCL1(2)). The SM showed narrow limits of agreement with CL2, a concordance correlation of 0.97, and a total deviation index of 14.73%. Similar results were obtained for the validation group. This protocol is reliable, reproducible, can be performed in conscious animals, uses a single dose of the marker, and requires a reduced number of samples, and avoids urine collection. Finally, it presents a significant improvement in animal welfare conditions and handling necessities in experimental trials.

• 出版日期2018-1