Anti-inflammatory properties of kaempferol via its inhibition of aldosterone signaling and aldosterone-induced gene expression

作者：Liu, Zi-Kui; Xiao, Hong-Bo^*; Fang, Jun

来源：Canadian Journal of Physiology and Pharmacology, 2014, 92(2): 117-123.

DOI：10.1139/cjpp-2013-0298

• aldosterone
• osteopontin
• cluster of differentiation 44
• nuclear factor kappa B
• kaempferol
• human umbilical vein endothelial cells

Osteopontin (OPN), also called cytokine Eta-1, is a pro-inflammatory cytokine. Recent studies have shown that aldosterone increases OPN gene expression in endothelial cells. As a flavonoid compound, kaempferol has potent anti-inflammatory properties, but whether kaempferol regulates aldosterone signaling and aldosterone-induced gene expression is still unknown. Human umbilical vein endothelial cells (HUVECs) were pretreated with kaempferol (0, 1, 3, or 10 mu mol/L) for 1 h prior to exposure to aldosterone (10(-6) mol/L) for 24 h. Aldosterone induced generation of reactive oxygen species; OPN and cluster of differentiation 44 gene expression; phospho-p38 MAPK and NF-kappa B binding activity. The effect of aldosterone was abrogated by kaempferol and spironolactone (10-6 mol/L). The present results suggest that kaempferol exerts its anti-inflammatory properties via its inhibition of aldosterone signaling and aldosterone-induced gene expression in HUVECs.

• 出版日期2014-2
• 单位湖南农业大学