A cDNA-AFLP approach to look for differentially expressed gene fragments in dioecious pointed gourd (Trichosanthes dioica Roxb.) for understanding sex expression

作者：Roy Subhash Kanti^*; Gangopadbyay Gaurab; Ghose Kaushik; Dey Sanjukta; Basu Debabrata; Mukherjee Kalyan Kumar

来源：Current Science, 2008, 94(3): 381-385.

• cDNA-AFLP
• gene fragments
• sex determination
• Trichosanthes dioica

Transcript profiling of unopened male and female floral buds of pointed gourd (Trichosanthes dioica Roxb.), a dioecious cucurbit, at uniform developmental stage was done through eDNA-AFLP to look for the differentially expressed unique and/or up-regulated gene fragments associated with sex expression. Thirty-one such fragments, twenty-three from male and eight from female, were selected, cloned, sequenced and assigned putative protein functions after database searching and obtaining GenBank accessions. The annotation of one male-derived clone (TDM16) as polygalacturonase with remarkably high level of homology as revealed from database searching is a promising finding, since this protein has direct involvement with pollen development, germination and tube growth with particular function in depolymerization of pectin.

• 出版日期2008-2-10