Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappa B1 DNA binding domain

作者：Lambert Cherie^*; Li Jimei; Jonscher Karen; Yang Teng Chieh; Reigan Philip; Quintana Megan; Harvey Jean; Freed Brian M

来源：Journal of Biological Chemistry, 2007, 282(27): 19666-19675.

DOI：10.1074/jbc.M611527200

Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha, beta-unsaturated aldehydes in cigarette smoke ( acrolein and crotonaldehyde) inhibited production of interleukin- 2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T cells but did not inhibit production of IL-8. The saturated aldehydes (acetaldehyde, propionaldehyde, and butyraldehyde) in cigarette smoke were inactive. Acrolein inhibited induction of NF-kappa B DNA binding activity after mitogenic stimulation of T cells but had no effect on induction of NFAT or AP-1. Acrolein inhibited NF-kappa B1 (p50) binding to the IL-2 promoter in a chromatin immunoprecipitation assay by > 99%. Using purified recombinant p50 in an electrophoretic mobility shift assay, we demonstrated that acrolein was 2000-fold more potent than crotonaldehyde in blocking DNA binding to an NF-kappa B consensus sequence. Matrix-assisted laser desorption/ionization time-of-flight and tandem mass spectrometry demonstrated that acrolein alkylated two amino acids (Cys-61 and Arg-307) in the DNA binding domain. Crotonaldehyde reacted with Cys-61, but not Arg-307, whereas the saturated aldehydes in cigarette smoke did not react with p50. These experiments demonstrate that aldehydes in cigarette smoke can regulate gene expression by direct modification of a transcription factor.

• 出版日期2007-7-6