科研之友
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摘要
We previously reported that chronic ethanol lowers blood pressure in female rats in this study hemodynamic biochemical and immunoblot analyses were performed to investigate (i) the roles of cardiac contractility and autonomic activity in the hypotensive action of ethanol and (ii) whether endotoxemia induced upregulation of cardiac and/or vascular nitric oxide synthase (NOS) isoforms underlies the hypotensive and cardiac effects of ethanol Telemetric monitoring of blood pressure heart rate and myocardial contractility (dP/dt(max)) was performed in female rats receiving liquid diet with or without ethanol (5% w/v 13 weeks) Autonomic control was assessed by frequency domain analysis of interbeat intervals (IBI) and systolic blood pressure (SBP) Compared with pair-fed controls ethanol caused sustained reductions in blood pressure heart rate and + dP/dt(max) Ethanol feeding increased the spectral power of high-frequency band (IBIHF 0 75-3 Hz) and decreased the low-frequency band (IBILF 025-0 75 Hz) and IBILF/HF ratio suggesting increased cardiac parasympathetic dominance In contrast vascular tone was not affected by ethanol because SBP spectral bands and plasma norepinephrine remained unchanged Myocardial expressions of eNOS and its upstream regulators phosphandylinositol 3-kinase (PI3K) and Akt and plasma endotoxin and nitrite/nitrate were increased by ethanol Mycardial iNOS was also increased by ethanol whereas nNOS remained unchanged and aortic levels of all NOS isoforms were not altered by ethanol These findings suggest that facilitation of myocardial PI3K/Akt/eNOS and iNOS pathways due possibly to ethanol induced endotoxemia and/or increased car
- 出版日期2011-1-10
