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摘要
Highly sensitive determination of proteins is essential to biomedical research and clinical diagnosis. Here a new fluorescence strategy for protein assays is reported that utilizes the exponential amplification reaction and silver nanocluster label-free architecture. Superior sensitivity was obtained for adenosine deaminase with a detection limit of 0.8 milliunit per liter. This method was employed for the determination of adenosine deaminase in human serum. The method displayed high selectivity for adenosine deaminase compared to other proteins, thus providing a promising alternative to standard approaches for the determination of proteins.
- 出版日期2015-8-13
- 单位南京医科大学
