Nonresonant background suppression for coherent anti-Stokes Raman scattering microscopy using a multi-wavelength time-lens source

作者：Li, Bo^*; Charan, Kriti; Wang, Ke; Rojo, Tomas; Sinefeld, David; Xu, Chris

来源：Optics Express, 2016, 24(23): 26687-26695.

DOI：10.1364/OE.24.026687

• HIGH-SENSITIVITY
• CARS SPECTROSCOPY
• RESOLUTION
• SYSTEM
• PULSES

We demonstrate a robust, all-fiber, two-wavelength time-lens source for background-free coherent anti-Stokes Raman scattering imaging. The time-lens source generates two picosecond pulse trains simultaneously: one at 1064 nm and the other tunable between 1040 nm and 1075 nm (similar to 400 mW for each wavelength). When synchronized to a mode-locked Ti: Sapphire laser, the two wavelengths are used to obtain on-and off-resonance coherent anti-Stokes Raman scattering images. Real-time subtraction of the nonresonant background in the coherent anti-Stokes Raman scattering image is achieved by the synchronization of the pixel clock and the time-lens source. Background-free coherent anti-Stokes Raman scattering imaging of sebaceous glands in ex vivo mouse tissue is demonstrated.

• 出版日期2016-11-14
• 单位深圳大学