A NOVEL BUCKWHEAT PROTEIN WITH A BENEFICIAL EFFECT IN ATHEROSCLEROSIS WAS PURIFIED FROM FAGOPYRUM TATARICUM (L.) GAERTN

作者：Zhou Xiaoli^*; Huang Lin; Tang Wen; Zhou Yiming; Wang Qing; Li Zongjie

来源：Archives of Biological Sciences, 2013, 65(2): 767-772.

DOI：10.2298/ABS1302767Z

• Buckwheat protein
• atherosclerosis
• hypocholesterolemic activity
• bile acid salt binding activity
• free radical scavenging activity

Buckwheat seeds contain many kinds of functional compounds that are of benefit to patients with cardiovascular disease. In this research, a water-soluble buckwheat protein was isolated and purified through a DEAE-Sepharose anion exchange column and Sephadex G-75 gel chromatography. The isolated buckwheat protein fractions exhibited hypocholesterolemic activity in a HepG2 cell model and demonstrated prominent bile acid salt-binding activity in an in vitro assay. The antioxidative activity of protein fractions with hypolipidemic effects was detected in a free radical scavenging experiment. The buckwheat protein fraction with the most obvious hypolipidemic activity and free radical scavenging activity was named as WSBWP. Its molecular weight was estimated by SDS-PAGE electrophoresis to be 38 kDa. It could become a potential candidate in the treatment of atherosclerosis.

• 出版日期2013
• 单位上海应用技术大学