A novel iron supplements preparation from Grifola frondosa polysaccharide and assessment of antioxidant, lymphocyte proliferation and complement fixing activities

作者：Xu, Lei; Meng, Yongbin; Liu, Ying; Meng, Qinghuan; Zhang, Zidong; Li, Jian; Lu, Qi^*

来源：International Journal of Biological Macromolecules, 2018, 108: 1148-1157.

DOI：10.1016/j.ijbiomac.2017.10.163

• Polysaccharide-iron (III) complex
• Antioxidant activity

Grifola frondosa polysaccharide-iron (III) complex (GFP-iron) was synthesized and characterized. Based on single factor and orthogonal optimization experiments of GFP-iron (III) complex synthesis, the optimum conditions were the reaction temperature 80 degrees C, pH 8, reaction time 90 min and ratio of catalyst to GFP 1:1.0 gg(-1). The iron content of GFP-iron (III) complex reached 24.15% under the optimums and characterized by fourier transform infrared (FTIR), scanning electron microscopy (SEM), X-Ray Diffraction (XRD), thermogravimetry (TG) and iron release in vitro assay.
By 5-axe cobweb charts, the antioxidant activity of GFP-iron (III) complex was comprehensively evaluated. The results showed GFP-iron (III) complex retained a certain antioxidant activity. The lymphocytes proliferation of GFP-iron (III) complex was increased by 61.68% comparing with that of GFP at the sample concentration of 62.5 mu g/mL. At giving 50% haemolysis, the concentration of GFP-iron (III) complex was 0.261 mg/mL. Therefore, GFP-iron (III) complex would be expected to exploit into a new-type comprehensive iron supplements.

• 出版日期2018-3
• 单位东北林业大学