Direct-Imaging-Based Quantification of Bacillus cereus ATCC 14579 Population Heterogeneity at a Low Incubation Temperature

作者:den Besten Heidy M W*; Garcia Diego; Moezelaar Roy; Zwietering Marcel H; Abee Tjakko
来源:Applied and Environmental Microbiology, 2010, 76(3): 927-930.
DOI:10.1128/AEM.01372-09

摘要

Bacillus cereus ATCC 14579 was cultured in microcolonies on Anopore strips near its minimum growth temperature to directly image and quantify its population heterogeneity at an abusive refrigeration temperature. Eleven percent of the microcolonies failed to grow during low-temperature incubation, and this cold-induced population heterogeneity could be partly attributed to the loss of membrane integrity of individual cells.

  • 出版日期2010-2