Maternal cigarette smoking during pregnancy is associated with downregulation of miR-16, miR-21 and miR-146a in the placenta

作者：Maccani Matthew A; Avissar Whiting Michele; Banister Carolyn E; McGonnigal Bethany; Padbury James F; Marsit Carmen J^*

来源：Epigenetics, 2010, 5(7): 583-589.

DOI：10.4161/epi.5.7.12762

• miRNA
• placenta
• cigarette smoking
• nicotine
• benzo(a)pyrene
• epigenetics

Maternal cigarette smoking during pregnancy is associated with poor fetal outcome and aberrant miRNA expression is associated with adverse pregnancy outcomes. In 25 human placentas, we analyzed the expression of four candidate miRNA previously implicated in growth and developmental processes: miR-16, miR-21, miR-146a and miR-182, and used three immortalized placental cell lines to identify if specific components of cigarette smoke were responsible for alterations to miRNA expression. miR-16, miR-21 and miR-146a were significantly downregulated in cigarette smoke-exposed placentas compared to controls. TCL-1 cells exposed to both nicotine and benzo(a)pyrene exhibited significant, dose-dependent downregulation of miR-146a. These results suggest that miR-146a is particularly responsive to exposures, and that smoking may elicit some of its downstream effects through alteration of miRNA expression.

• 出版日期2010-10-1