Accumulation of FGF9 in Prostate Cancer Correlates with Epithelial-to-Mesenchymal Transition and Induction of VEGF-A Expression

作者：Teishima Jun^*; Yano Shigeki; Shoji Koichi; Hayashi Tetsutaro; Goto Keisuke; Kitano Hiroyuki; Oka Kiyotaka; Nagamatsu Hirotaka; Matsubara Akio

来源：Anticancer Research, 2014, 34(2): 695-700.

• FGF9
• prostate cancer
• VEGF-A
• N-cadherin
• epithelial-mesenchymal transition
• biochemical recurrence
• radical prostatectomy

Aim: The aim of the present study was to investigate the molecular mechanism of fibroblast growth factor (FGF)-9 in prostate cancer cells. Materials and Methods: Expression of vascular endothelial growth factor (VEGF)s and cadherins in LNCaP cells by incubation with FGF9 was assessed by western blot analysis. Tissues obtained during a radical prostatectomy in 88 patients were immunohistochemically-stained using anti-FGF9, anti-cadherin, and anti-VEGF antibodies. Results: Expression of N-cadherin and VEGF-A were induced in LNCaP cells incubated in FGF9-containing medium. The biochemical relapse-free survival rate in cases with FGF9, N-cadherin and VEGF-A-positive cells was significantly lower than the rate in cases where positive cells were not detectable. The prevalence of both VEGF-A- and N-cadherin-positive cells in the sample with FGF9-positive cells were significantly higher in comparison to FGF9-negative cases. Conclusion: FGF9 can be associated with epithelial-to-mesenchymal transition and invasion by inducing VEGF-A expression in prostate cancer cells.

• 出版日期2014-2