New PCR Test for Detection of Candida glabrata Based on the Molecular Target Chosen by the RAPD Technique

作者：Olchawa Anna; Krawczyk Beata; Brillowska Dabrowska Anna^*

来源：Polish Journal of Microbiology, 2013, 62(1): 81-84.

• Candida glabrata
• molecular targets
• PCR
• RAPD
• real-time PCR

Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.

• 出版日期2013