An efficient and reproducible process for transmission electron microscopy (TEM) of rare cell populations

作者：Kumar Sachin^*; Ciraolo Georgianne; Hinge Ashwini; Filippi Marie Dominique

来源：Journal of Immunological Methods, 2014, 404: 87-90.

DOI：10.1016/j.jim.2013.11.025

• Transmission electron microscopy
• Ultra structure
• Rare population
• Stem cell

Transmission electron microscopy (TEM) provides ultra-structural details of cells at the sub-organelle level. However, details of the cellular ultrastructure, and the cellular organization and content of various organelles in rare populations, particularly in the suspension, like hematopoietic stem cells (HSCs) remained elusive. This is mainly due to the requirement of millions of cells for TEM studies. Thus, there is a vital requirement of a method that will allow TEM studies with low cell numbers of such rare populations. We describe an alternative and novel approach for TEM studies for rare cell populations. Here we performed a TEM study from 10,000 HSC cells with relative ease. In particular, tiny cell pellets were identified by Evans blue staining after PFA-GA fixation. The cell pellet was pre-embedded in agarose in a small microcentrifuge tube and processed for dehydration, infiltration and embedding. Semi-thin and ultra-thin sections identified clusters of numerous cells per sections with well preserved morphology and ultrastructural details of golgi complex and mitochondria. Together, this method provides an efficient, easy and reproducible process to perform qualitative and quantitative TEM analysis from limited biological samples including cells in suspension.

• 出版日期2014-2