Absence of polo-like kinase 3 in mice stabilizes Cdc25A after DNA damage but is not sufficient to produce tumors

作者：Myer David L; Robbins Susan B; Yin Moying; Boivin Gregory P; Liu Yang; Greis Kenneth D; Bahassi El Mustapha; Stambrook Peter J^*

来源：Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2011, 714(1-2): 1-10.

DOI：10.1016/j.mrfmmm.2011.02.006

• Polo-like kinases
• Cdc25A
• DNA damage
• Checkpoints
• Ubiquitinylation

The polo-like kinases (Plks1-5) are emerging as an important class of proteins involved in many facets of cell cycle regulation and response to DNA damage and stress. Here we show that Plk3 phosphorylates the key cell cycle protein phosphatase Cdc25A on two serine residues in its cyclinB/cdk1 docking domain and regulates its stability in response to DNA damage. We generated a Plk3 knock-out mouse and show that Cdc25A protein from Plk3-deficient cells is less susceptible to DNA damage-mediated degradation than cells with functional Plk3. We also show that absence of Plk3 correlates with loss of the G1/S cell cycle checkpoint. However, neither this compromised DNA damage checkpoint nor reduced susceptibility to proteasome-mediated degradation after DNA damage translated into a significant increase in tumor incidence in the Plk3-deficient mice.

• 出版日期2011-9-1