Adaptive immune responses against parvovirus B19 in patients with myocardial disease

作者：Lindner Juha; Noutsias Michel; Lassner Dirk; Wenzel Juergen; Schultheiss Heinz Peter; Kuehl Uwe; Modrow Susanne^*

来源：Journal of Clinical Virology, 2009, 44(1): 27-32.

DOI：10.1016/j.jcv.2008.09.007

• Parvovirus B19
• Cellular immune response
• Humoral immune response
• Cardiomyopathy
• Myocarditis

Background: Parvovirus B19 (B19V)-DNA is frequently detected in endomyocardial biopsies (EMBs) from patients with acute myocarditis (AMC) and dilated cardiomyopathy (DCM), but also in various healthy tissues. The clinical relevance of this DNA-persistence is unclear.
Objectives: To investigate potential pathogenic influences of B19V-DNA in EMBs, we analyzed B19V-specific adaptive immune responses in AMC/DCM patients and healthy controls.
Study design: 15 AMC/DCM patients with detectable B19V-DNA in EMBs and 51 controls were analyzed for signs of acute B19V-infections and virus-specific immune responses by PCR, ELISA, Western line, and ELISpot-assays.
Results: Productive B19V-infection was determined in three patients. Slightly lower levels of B19V-specific T-cells were observed in patients as compared to the controls, no differences were observed in virus-specific serology. Viral DNA-load in EMBs could not be correlated to the number of B19V-specific T-cells. No differences in T-cell response, viremia and/or serological markers indicative for viral pathogenesis were observed in patients with inflammatory cardiomyopathy.
Conclusions: Discrepancies in B19V-specific adaptive immunity were not observed in AMC/DCM patients as compared to controls. The data indicate that the exclusive detection of B19V-DNA in EMBs is not sufficient to associate B19V with AMC/DCM but should be complemented with additional virological and immunological parameters in further studies.

• 出版日期2009-1
• 单位河北医科大学