A simple and economic method for simultaneous determination of 11 antibiotics in manure by solid-phase extraction and high-performance liquid chromatography

作者：Feng, Yao; Wei, Chaojun; Zhang, Wenjuan; Liu, Yuanwang; Li, Zhaojun^*; Hu, Haiyan; Xue, Jianming; Davis, Murray

来源：Journal of Soils and Sediments, 2016, 16(9): 2242-2251.

DOI：10.1007/s11368-016-1414-5

• Antibiotics
• Determination
• High-performance liquid chromatography
• Manures
• Solid-phase extraction

A simple and highly efficient economic method for the analysis of 11 antibacterial drugs including two tetracyclines, three quinolones, four sulfonamides, chloramphenicol and tylosin, in livestock manure, was developed using solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC).
The analytes were successively extracted by EDTA-McIlvaine solution and organic solvent mixture. The extracts were degreased with n-hexane and cleaned through SPE on a hydrophile-lipophile balance (HLB) cartridge. All compounds were determined on a C18 reverse phase column with gradient elution.
Recoveries calculated from spiked samples of animal manures ranged from 62.65 to 99.16 % for 11 antibiotics with relative standard deviations of less than 10.0 %. Limits of detection ranged from 0.1 to 1.9 mu g kg(-1), and limits of quantification ranged from 0.3 to 5.9 mu g kg(-1).
The results show that SPE-HPLC is an inexpensive and practical method for rapid detection of multiple antibiotics in animal manure.

• 出版日期2016-9
• 单位中国农业科学院农业资源与农业区划研究所; 北京农学院; 山西师范大学