Discovery of Lipids from B. longum subsp infantis using Whole Cell MALDI Analysis

作者：Timmer Mattie S M^*; Sauvageau Janelle; Foster Amy J; Ryan Jason; Lagutin Kirill; Shaw Odette; Harper Jacquie L; Sims Ian M; Stocker Bridget L

来源：Journal of Organic Chemistry, 2014, 79(16): 7332-7341.

DOI：10.1021/jo501016c

Bifidobacteria are dominant members of the microbial community in the intestinal tract of infants, and studies have shown that glycolipids extracted from the cell surface of these bacteria elicit beneficial immune responses. Accordingly, the identification and structural characterization of glycolipids from the cell wall of bifidobacteria is the first step in correlating glycolipid structure with biological activity. Using whole cell MALDI as a screening tool, we herein present for the first time the identification and structural elucidation of the major polar lipids from Bifidobacterium longum subs. infantis. The lipids identified include an unprecedented plasmenyl cyclophosphatidic acid and a mixed acetal glycolipid, with the latter subsequently being isolated and found to suppress the innate immune response.

• 出版日期2014-8-15
• 单位河北医科大学