Genetic diversity assessment of wheat (Triticum aestivum L.) landraces from west of Urmia lake in Iran by RAPD markers

作者：Chaparzadeh N; Dolati M; Aftabi Y; Miandoab L Zarandi

来源：Agrochimica, 2010, 54(5): 279-288.

• genetic diversity
• Iran
• RAPD markers
• wheat landraces

Genetic diversity in 18 hexaploid wheat (Triticum aestivum L.) landraces from northwest of Iran (west of Urmia lake) was analysed using Random Amplified Polymorphic DNA (RAPD) molecular markers, a polymerase chain reaction (PCR) based technique. With 20 selected RAPD primers, from 121 amplified bands 100 were polymorphic (84.46%). Polymorphic Information Content (PIC) values were calculated for each primer. Genetic diversity of all landraces estimated by Nei's genetic diversity ranged from 0.000 to 0.498 and Shannon diversity index ranged from 0.000 to 0.691. The clustering algorithm divided the varieties into three major groups. Results revealed that RAPD markers could be efficiently used to quickly differentiate local landraces obtained from different locations. The information on genetic similarity will be useful to establish a germplasm bank of wheat landraces and to develop breeding programmes.

• 出版日期2010-10