Involvement of MAK-1 and MAK-2 MAP kinases in cell wall integrity in Neurospora crassa

作者：Kamei Masayuki; Yamashita Kazuhiro; Takahashi Masakazu; Fukumori Fumiyasu; Ichiishi Akihiko; Fujimura Makoto^*

来源：Bioscience Biotechnology and Biochemistry, 2016, 80(9): 1843-1852.

DOI：10.1080/09168451.2016.1189321

• cell wall integrity
• MAP kinase
• micafungin
• Neurospora crassa

Among three MAPK disruptants of Neurospora crassa, mak-1 was sensitive and mak-2 was hypersensitive to micafungin, a beta-1,3-glucan synthase inhibitor, than the wild-type or os-2 strains. We identified six micafungin-inducible genes that are involved in cell wall integrity (CWI) and found that MAK-1 regulated the transcription of non-anchored cell wall protein gene, ncw-1, and the beta-1,3-endoglucanase gene, bgt-2, whereas MAK-2 controlled the expression of the glycosylhydrolase-like protein gene, gh76-5, and the C4-dicarboxylate transporter gene, tdt-1. Western blotting analysis revealed that, in the wild-type strain, MAK-1 was constitutively phosphorylated from conidial germination to hyphal development. In contrast, the phosphorylation of MAK-2 was growth phase-dependent, and micafungin induced the phosphorylation of unphosphorylated MAK-2. It should be noted that the phosphorylation of MAK-1 was virtually abolished in the mak-2 strain, but was significantly induced by micafungin, suggesting functional cross talk between MAK-1 and MAK-2 signalling pathway in CWI.

• 出版日期2016-9