A Molecular Method to Correlate Bloodstains with Wound Site for Crime Scene Reconstruction

作者：Johnson Donald J^*; Andersen Cheryl; Scriven Katherine A; Klein Amberly N; Choi Mo Re; Carroll Cindy; de Leon Ray D

来源：Journal of Forensic Sciences, 2014, 59(3): 735-742.

DOI：10.1111/1556-4029.12377

• forensic biology
• animal model
• bloodstain patterns
• crime scene reconstruction
• forensic science
• microRNA
• criminalistics
• wounds

Bloodstain pattern analysis to determine the wound-of-origin of bloodstains is problematic with nonspecific patterns. In this proof-of-concept study, the authors examined a molecular approach to correlate bloodstains with injuries using the rat as a model. Specifically, investigations were conducted on the rat brain marker, rno-miR-124-3p, with the QIAGEN miScript System and real-time PCR analysis. Rno-miR-124-3p was detected in brain homogenates diluted 100,000 times; in 3-week-old, room temperature stored, simulated brain-blood stains; and in bloodstains from head gunshot wounds collected with swabs and subsequently frozen for 9-18months; however, rno-miR-124-3p was not detected in whole blood. Proof-of-principle was demonstrated by the ability to distinguish bloodstains from a gunshot wound to the head versus bloodstains from a gunshot wound to the chest, by the testing of otherwise identical bloodstains from the two patterns for the presence of the marker. The results suggest a viable approach to a longstanding problem in casework.

• 出版日期2014-5