Molecular Imaging of Cell Death in an Experimental Model of Parkinson%26apos;s Disease with a Novel Apoptosis-Targeting Peptide

作者：Lee Min Jeong; Wang Kai; Kim In San; Lee Byung Heon^*; Han Hyung Soo

来源：Molecular Imaging and Biology, 2012, 14(2): 147-155.

DOI：10.1007/s11307-011-0497-z

• Parkinson%26apos
• s disease
• Apoptosis-targeting peptide
• In vivo imaging
• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• Apoptosis

We used a novel apoptosis-targeting peptide called ApoPep-1 in order to evaluate whether ApoPep-1 can be used as a diagnostic indicator in a model of Parkinson%26apos;s disease (PD). %26lt;br%26gt;1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was given to mice to produce the PD model. Cy7.5-labeled ApoPep-1 was given intravenously and optical imaging was taken at 1, 2, and 3 weeks after MPTP treatment. Immunohistochemical study was performed with brain sections. %26lt;br%26gt;Increased ApoPep-1 signal was observed in the brain of MPTP-treated mice by in vivo and ex vivo imaging study. With histological evaluation, ApoPep-1 signal demonstrated a strong correlation with loss of dopaminergic neurons or increase of apoptotic cells. Moreover, the neuroprotective effect of amantadine in the MPTP model was effectively evaluated using optical imaging of ApoPep-1. %26lt;br%26gt;We conclude that ApoPep-1 is the effective probe for imaging of apoptosis in the MPTP model and can be applied in brain diseases with apoptosis.

• 出版日期2012-4