Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples

作者：Li, Li^*; Xia, Li-Ru; Zhao, Yong-Fu; Wang, He-Ye

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2016, 1029: 72-80.

DOI：10.1016/j.jchromb.2016.07.012

• Mycotoxins
• UV-initiated copolymerization
• Immune-affinity array
• Chemiluminescent detection
• Monolith

In this paper, a novel highly sensitive chemiluminescence immune-affinity 96 spots monolith array was developed to detect deoxynivalenol (DON), zearalenone (ZEN), T-2 toxin (T-2), and fumonisin B-1 (FB1) in corn samples. Firstly, the monolith array was prepared through on suit UV-initiated copolymerization using polyethylene glycol diacrylate (PEGDA) as cross-linker, glycidyl methacrylate (GMA) as functional monomer and polyethylene glycol 200 (PEG 200) as the porogen. Subsequently, the four mycotoxins immune-affinity monolith array was prepared by immobilization of DON, ZEN, T-2, and FBI antibody. The mole ratio of PEGDA/GMA, UV exposure time, and the volume ratio of PEG 200/PEGDA were optimized to improve the performances of the immune-affinity monolith array. For the mycotoxins immune-affinity monolith array based on chemiluminescence detection, the limit of detection was 0.0036 ng/mL (DON), 0.0048 ng/mL (ZEN), 0.0039 ng/mL (T-2), and 0.0017 ng/mL (FB1), respectively. The linear response in the range of 0.01-0.1 ng/mL (R-2 = 0.98). The results showed that the proposed four mycotoxins immune affinity monolith array was a stable, accurate, and highly sensitive method to determine levels of DON, ZEN, T-2, and FB1 in real samples.

• 出版日期2016-9-1
• 单位江苏省农业科学院