An improved smaller biotin ligase for BioID proximity labeling

作者:Kim, Dae In; Jensen, Samuel C.; Noble, Kyle A.; Birendra, K. C.; Roux, Kenneth H.; Motamedchaboki, Khatereh; Roux, Kyle J.*
来源:Molecular Biology of the Cell, 2016, 27(8): 1188-1196.
DOI:10.1091/mbc.E15-12-0844

摘要

The BioID method uses a promiscuous biotin ligase to detect protein-protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fusion proteins, requires less biotin supplementation, and exhibits enhanced labeling of proximate proteins. Thus BioID2 improves the efficiency of screening for protein-protein associations. We also demonstrate that the biotinylation range of BioID2 can be considerably modulated using flexible linkers, thus enabling application-specific adjustment of the biotin-labeling radius.

  • 出版日期2016-4-15