Water in oil based PGPR formulation of Pseudomonas fluorescens (FP7) showed enhanced resistance against Colletotrichum musae

作者:Peeran Mohammed Faisal*; Nagendran Krishnan; Gandhi Karthikeyan; Raguchander Thiruvengadam; Prabakar Kuppusami
来源:Crop Protection, 2014, 65: 186-193.
DOI:10.1016/j.cropro.2014.07.010

摘要

Banana anthracnose incited by Colletotrichum musae is a serious disease in post harvest marketing stage. It occurs in almost all the banana growing countries. In the current study, 5 isolates of C. musae infecting banana cv nendran were collected from various district of Tamil Nadu. C5 isolate collected from Tiruchirappalli showed maximum virulent with (66.60%) PDI. In vitro efficacy of six Pseudomonas fluorescens superior strains were tested for their inhibition on mycelial growth of C musae. P. fluorescens (FP7) showed significantly higher inhibition of 41.12 per cent over control and with a mean mycelial growth of 52.10 mm. Water in oil emulsion was used in the present research for formulation of P. fluorescens (FP7). The emulsion contained the following ingredients (w/w), sterile deionized water (45.25%), glycerine (4.00%), water-soluble wax ((0.75%), Tween 20 (2.50%), and a mixture of 19.00% soybean oil + 28.50% castor oil. The application of water in oil emulsion formulation significantly increased the yield (68.12 t/ha) compared to control (66.49 t/ha), and also increase in the activity of defense related enzymes viz., phenyl alanine ammonia lyase, peroxidase, polyphenol oxidase, catalase and beta-1, 3 glucanase was observed which was followed by talc based formulation of Pf1 and FP7.

  • 出版日期2014-11