Comparative Genomic and Regulatory Analyses of Natamycin Production of Streptomyces lydicus A02

作者：Wu, Huiling; Liu, Weicheng^*; Shi, Lingling; Si, Kaiwei; Ting Liu; Dan Dong; Zhang, Taotao; Juan Zhao; Liu, Dewen; Tian, Zhaofeng; Yue, Yuesen^*; Hong Zhang; Bai Xuelian; Yong Liang

来源：Scientific Reports, 2017, 7(1): 9114.

DOI：10.1038/s41598-017-09532-3

• POLYENE MACROLIDE PIMARICIN
• PHOR-PHOP SYSTEM
• POSITIVE REGULATOR
• MORPHOLOGICAL-DIFFERENTIATION
• ANTIBIOTIC PRODUCTION
• FUNCTIONAL-ANALYSIS
• CONJUGAL TRANSFER
• ESCHERICHIA-COLI
• SEQUENCE
• BIOSYNTHESIS

Streptomyces lydicus A02 is used by industry because it has a higher natamycin-producing capacity than the reference strain S. natalensis ATCC 27448. We sequenced the complete genome of A02 using next-generation sequencing platforms, and to achieve better sequence coverage and genome assembly, we utilized single-molecule real-time (SMRT) sequencing. The assembled genome comprises a 9,307,519-bp linear chromosome with a GC content of 70.67%, and contained 8,888 predicted genes. Comparative genomics and natamycin biosynthetic gene cluster (BGC) analysis showed that BGC are highly conserved among evolutionarily diverse strains, and they also shared closer genome evolution compared with other Streptomyces species. Forty gene clusters were predicted to involve in the secondary metabolism of A02, and it was richly displayed in two-component signal transduction systems (TCS) in the genome, indicating a complex regulatory systems and high diversity of metabolites. Disruption of the phoP gene of the phoR-phoP TCS and nsdA gene confirmed phosphate sensitivity and global negative regulation of natamycin production. The genome sequence and analyses presented in this study provide an important molecular basis for research on natamycin production in Streptomyces, which could facilitate rational genome modification to improve the industrial use of A02.

• 出版日期2017-8-22
• 单位北京市农林科学院; 深圳华大生命科学研究院