Development of a downscale sedimentation field flow fractionation device for biological event monitoring

作者：Begaud Grimaud G; Battu S^*; Liagre B; Beneytout J L; Jauberteau M O; Cardot P J P

来源：Journal of Chromatography A, 2009, 1216(52): 9125-9133.

DOI：10.1016/j.chroma.2009.08.042

• Downscale device
• Sedimentation field flow fractionation
• Monitoring
• Cell(s)
• Apoptosis
• Differentiation

Classically described as a macroscale size-density based method, Sedimentation field flow fractionation (SdFFF) has been successfully used for cell sorting. The goal of this study was to develop a new SdFFF device for downscale applications, in particular for oncology research to rapidly monitor chemical biological event induction in a cell line. The development of a downscale SdFFF device required reduction of the separation channel Volume. Taking advantage of a newly laboratory designed apparatus, channel volume was successfully decreased by reducing both length and breadth. To validate the apparatus and method, we used the well-known model of diosgenin dose-dependent induction of apoptosis or megakaryocytic differentiation in HEL cells. After a minute scale acquisition of a reference profile, the downscale device was able to perform fast, early, significant and reproducible monitoring of apoptosis and differentiation, two important biological mechanisms in the field of cancer research.

• 出版日期2009-12-25