DELIVERY OF TFPI-2 USING ULTRASOUND WITH A MICROBUBBLE AGENT (SONOVUE) INHIBITS INTIMAL HYPERPLASIA AFTER BALLOON INJURY IN A RABBIT CAROTID ARTERY MODEL

作者：Zhou, Jie; Wang, Yuxue; Xiong, Yufang; Wang, Hongxing; Feng, Youmei; Chen, Juan^*

来源：Ultrasound in Medicine and Biology, 2010, 36(11): 1876-1883.

DOI：10.1016/j.ultrasmedbio.2010.08.013

• TFPI-2
• Intimal hyperplasia
• Ultrasound
• Carotid artery
• SonoVue

Here we report a new, simple and efficient method by using ultrasound and a microbubble agent (SonoVue) for delivering a gene to balloon-injured carotid arteries for restenosis prophylaxis. The tissue factor pathway inhibitor-2 (TFPI-2) has been shown to inhibit the postinjury intimae hyperplasia in atherosclerotic vessels. New Zealand white rabbits were divided into 4 groups with 14 in each, a treatment control for balloon injury, a gene vehicle control, a gene delivery of TFPI-2 without using ultrasound and a gene delivery of TFPI-2 using ultrasound. After four weeks, the injured artery neointimal proliferation was significantly lower in the TFPI-2 group with ultrasound than the control groups (p<0.01) according to the measurement of the mean luminal diameters by B-mode ultrasonography. The ratio of intimal/media area and the stenosis rate in the gene delivery facilitated by ultrasound were significantly lower than those of the nonultrasound gene delivering method (p<0.01). (E-mail: chenjuanlinda@gmail.

• 出版日期2010-11
• 单位华中科技大学