A method for regenerating gold surface for prolonged reuse of gold-coated surface plasmon resonance chip

作者：Vashist Sandeep Kumar^*

来源：Analytical Biochemistry, 2012, 423(1): 23-25.

DOI：10.1016/j.ab.2011.12.045

• Regeneration
• Gold surface
• Surface plasmon resonance
• Piranha solution
• APTES
• EDC
• Sulfo-NHS
• Human fetuin A immunoassay

We developed a method to completely regenerate the gold (Au) surface of 3-aminopropyltriethoxysilane (APTES)-functionalized Au-coated surface plasmon resonance (SPR) chip that had been used for human fetuin A (HFA) immunoassay. It involved treatment of the used SPR chip with freshly prepared piranha solution (concentrated H2SO4/30% H2O2 = 3:1, v/v) for 15 min followed by extensive rinsing with ethanol and deionized water. The developed method enabled prolonged reuse of the regenerated SPR chip that increased its cost-effectiveness without affecting the reproducibility of HFA immunoassays.

• 出版日期2012-4-1