Direct Targeted Quantitative Molecular Imaging of Neurotransmitters in Brain Tissue Sections

作者：Shariatgorji Mohammadreza; Nilsson Anna; Goodwin Richard J A; Kallback Patrik; Schintu Nicoletta; Zhang Xiaoqun; Crossman Alan R; Bezard Erwan; Svenningsson Per; Andren Per E^*

来源：Neuron, 2014, 84(4): 697-707.

DOI：10.1016/j.neuron.2014.10.011

Current neuroimaging techniques have very limited abilities to directly identify and quantify neurotransmitters from brain sections. We have developed a molecular-specific approach for the simultaneous imaging and quantitation of multiple neurotransmitters, precursors, and metabolites, such as tyrosine, tryptamine, tyramine, phenethylamine, dopamine, 3-methoxytyramine, serotonin, GABA, glutamate, acetylcholine, and L-alpha-glycerylphosphorylcholine, in histological tissue sections at high spatial resolutions. The method is employed to directly measure changes in the absolute and relative levels of neurotransmitters in specific brain structures in animal disease models and in response to drug treatments, demonstrating the power of mass spectrometry imaging in neuroscience.

• 出版日期2014-11-19